Table 1. Recommended and measured melting rates on high-resolution melting Instruments.
InstrumentRecommended instrument settingMeasured ramp rate (°C/s)aTurnaround time (min)Batch sizeThroughput (samples/h)
HR-10.3 °C/s0.31.5140
LS320.3 °C/s0.32.53224
LS960.1 °C/s0.15961152
Eco0.1 °C/s0.08748411
LC48025 acquisitions/°C0.04996640
Pikostep (0.04 °C)/hold (1 s)0.014096144
Rotor36/72ramp (0.1 °C)/hold (2 s)0.014036/7254/108
CFX96step (0.2 °C)/hold (10 s)0.015096115
StepOnePlus0.3% ramp0.005959661
  • a Measured with a microthermocouple or by temperature-sensitive fluorescence [Sanford and Wittwer (17)].