Table 1.

Advanced lipoprotein testing methods involved in the cross-platform comparison.

MethodsLaboratoryCovered measurandsCalibrators
ES-DMA-1 (27)Quest Diagnosticsnon-HDL-P, LDL-P, HDL-P, LDL size, full lipoprotein number concentration profileNone
ES-DMA-2 (28)LNEnon-HDL-P, LDL-P, LDL, and HDL sizeWHO/IFCC SP3-08
IN (25)NWLMDRLa (University of Washington)apoB-100WHO/IFCC SP3-08
LC-MS/MS-1A (offline digestion) (32)
LC-MS/MS-1B (online digestion) (31)
CDCapoA-I, apoA-II, apoA-IV, apoB-100, apoC-I, apoC-II, apoC-III, apoEPeptide calibrators, value assigned by amino-acid analysis by isotope dilution LC-MS/MS
LC-MS/MS-2A (33)LUMCapoA-I, apoB-100, apoC-I, apoC-II, apoC-III, apoESerum-based calibrators (CLSIb C37A), value assigned by the IN designated comparison method, calibrated with WHO/IFCC SP3-08
LC-MS/MS-2B (34)University of WashingtonapoA-I, apoB-100Native human serum samples, value-assigned by the IN designated comparison method, calibrated with WHO/IFCC SP3-08
NMR (LP3 software) (29)LabCorp - NIHnon-HDL-P; 3 VLDL-P, 3 LDL-P, 3 HDL-P subclasses; VLDL-P, LDL-P, HDL-P, sizesNone
NMR (LP4 software) (30)LabCorp - NIHnon-HDL-P; 5 TRL-Pc, 3 LDL-P, 7 HDL-P subclasses; TRL-P, LDL-P, HDL-P, sizesNone
VAP (35)VAP DiagnosticsdapoB-100, LDL-P, cholesterol distribution profileCholesterol profile: frozen serum from Solomon Park Research Laboratories, value assigned for cholesterol by the CEQALe
  • a Northwest Lipid Metabolism and Diabetes Research Laboratories.

  • b Clinical Laboratory Standard Institute.

  • c Triglyceride-rich lipoprotein particle number.

  • d Formerly Atherotech.

  • e Canadian External Quality Assessment Laboratory (Vancouver, BC, Canada).