Table 2.

LC-MS/MS univariate and multivariate linear regression coefficients, intercepts, and coefficients of determination for each IgG subclass with statistical parameters displayed separately for the respective IN IgG subclass methods.

Univariate regressionBSIN subclassSIN subclass
IgG1IgG2IgG3IgG4IgG1IgG2IgG3IgG4
N7373737340383840
Slope0.920.921.080.940.950.650.531.64
Intercept, g/L1.2a,b3.2c,d−0.020.020.422.4c,e0.110.13
R20.920.180.960.960.950.460.870.96
LC-MS/MS multivariate linear regression coefficients by immunonephelometric method
IgG1IgG2IgG3IgG4IgG1IgG2IgG3IgG4
IgG1 coefficient0.89f,g0.060.000.03a,h1.00f,i−0.020.000.07a,j
IgG2 coefficient0.010.89f,k−0.020.03−0.100.72f,l0.00−0.02
IgG3 coefficient−0.390.171.07f,m−0.30−1.39a,n0.010.53f,o−1.01a,p
IgG4 coefficient0.40f,q0.94f,r−0.010.94f.s0.110.24f,t0.001.65f,u
Intercept, g/L0.33−0.350.030.030.941.21a,v0.110.30
R20.970.870.970.960.960.740.870.98
  • a Significantly different from 0 (P < 0.05) but insignificant with robust linear regression;

  • b 0.032;

  • c significantly different from 0 (P < 0.05);

  • d 0.0041;

  • e 1.2 × 10−5;

  • f significantly different from 0 (P < 0.00001);

  • g 8.6 × 10−47;

  • h 0.044;

  • i 3.3 × 10−25;

  • j 0.0057;

  • k 5.5 × 10−10;

  • l 1.2 × 10−9;

  • m 3.2 × 10−47;

  • n 0.0026;

  • o 4.2 × 10−15;

  • p 0.0011;

  • q 2.0 × 10−15;

  • r 4.9 × 10−26;

  • s 1.1 × 10−48;

  • t 1.6 × 10−6;

  • u 1.6 × 10−29;

  • v 0.020.