Table 1. Metabolomics technologies.
ChromatographyNoneGC• Ideal for volatile nonpolar analytes
LC• Ideal for ionizable analytes in solution
Other• Direct infusion (no chromatography)
• Capillary electrophoresis
• Thin-layer chromatography
Analytical methodNMRMS
TOFTriple quadrupoleIon trap
Metabolite identificationChemical shiftm/z (Flight time)m/z (Filters for m/z)m/z (Trapping frequency)
Advantages• Robust• Mass accuracy• Sensitivity• Sensitivity
• No sample destruction• Wide mass range• Can perform tandem MS• Can perform tandem MS
• No chromatography or analyte ionization• Dynamic range
• Unambiguous identification of abundant analytes
Disadvantages• Limited sensitivity• Limited dynamic range• Poor mass accuracy• Limited dynamic range
• Isotopic standards required for absolute quantitation• Isotopic standards required for absolute quantitation• Isotopic standards required for absolute quantitation