Table 1.

Padlock probe, amplification primer, and array hybridization control sequences (5′–3′).1

Padlock probe5′ SegmentGeneral amplification sequenceTag3′ Segment
 Vβ 1agggaactgttgtgcgtagaAGAGTGTACCGACCTCAGTAGCCGTGACTATCGACTGTGCCTCGTTCTCGTGTAGAtctagttcagagtgcaagtc
 Vβ 2gtcaggcttgcatggttgatAGAGTGTACCGACCTCAGTAGCCGTGACTATCGACTGTTGACCGTTAGTTATGCGAttactttcagagtggacaag
 Vβ 3tgtacccctcagtaaaatctAGAGTGTACCGACCTCAGTAGCCGTGACTATCGACTATTTGACGAACGTATGCCGCattcttttctctagagacac
 Vβ 4tggatcgtcagggaagttcaAGAGTGTACCGACCTCAGTAGCCGTGACTATCGACTGCGTGTGTGGACTCTCTCTAttttgctatcgacttgacac
 Vβ 5.1aagtattcaaagaggaaatgAGAGTGTACCGACCTCAGTAGCCGTGACTATCGACTCTGGAATGTGACCGTGCTCTtgtctctctgtgtctcactg
 Vβ 5.2ctcagagctatagttatggaAGAGTGTACCGACCTCAGTAGCCGTGACTATCGACTGCACTAACTGGTCTGGGTCAaataaggcgttcacattcag
 Vβ 6tgactgcaaacaaccgatctAGAGTGTACCGACCTCAGTAGCCGTGACTATCGACTCTCACTAATCGTCTGCGGTGaaagatggatccctcaggcc
 Vβ 7tgttggggcatttaggtgatAGAGTGTACCGACCTCAGTAGCCGTGACTATCGACTATCCGGTCTCATCGCTGAATttgaaggaataagtgagagc
 Vβ 8aacgttgttgttaaagtaaaAGAGTGTACCGACCTCAGTAGCCGTGACTATCGACTTAGGACTTGCGTCTCTGTGCttccctgaatcatctatcgg
 Vβ 9gctttgtctggagatttagtAGAGTGTACCGACCTCAGTAGCCGTGACTATCGACTCGAGTGCTCCGTGCGAAATAtgatgtgaagatttaagtga
 Vβ 11tttgagaacatttcagagttAGAGTGTACCGACCTCAGTAGCCGTGACTATCGACTTAGCTCAGTTGTCCGGATCTtattttgtcattgcccatgg
 Vβ 12ttctcctttgccagtgtcttAGAGTGTACCGACCTCAGTAGCCGTGACTATCGACTTACCGCGCTTGGGACATGATacactatagccatctgagac
 Vβ 13tgtaccctcaccaattgattAGAGTGTACCGACCTCAGTAGCCGTGACTATCGACTGTCCtACATGGTGCTTCGTAtggatctctcctttggcagt
 Vβ 14gtatcttgggttctgggttaAGAGTGTACCGACCTCAGTAGCCGTGACTATCGACTCTGCCGCTGGGTCCAATATAttttcagttactgtgatgag
 Vβ 15tcgagagacattgtatccaaAGAGTGTACCGACCTCAGTAGCCGTGACTATCGACTTGCGTCTATTTAGTGGAGCCgagaatttagcctgtgcctg
 Vβ 16catgtccagaaattgtgtcaAGAGTGTACCGACCTCAGTAGCCGTGACTATCGACTCTGCCTGTGACTCGTGTATCtcgaaaccaataaagattat
 Vβ 17ttctgaaagtcatttattatAGAGTGTACCGACCTCAGTAGCCGTGACTATCGACTTGTCTATGTTTACAGCGGGCatccttcatctatatctcct
 Vβ 18tgcatctcagtcattcctccAGAGTGTACCGACCTCAGTAGCCGTGACTATCGACTCTTCTGATGATCTATGCGCGactgtgtcctttcattgggc
 Vβ 19tatgtcctttttctggagtaAGAGTGTACCGACCTCAGTAGCCGTGACTATCGACTCTGGACCTTAATCGTGTGCGttgataccaataaacaaaag
 Vβ 20ctcagagctgatcaggccatAGAGTGTACCGACCTCAGTAGCCGTGACTATCGACTATCTGTCCGACGCGAGATATtctgagagattctggggcac
 Vβ 21ccagatataggattgcaccaAGAGTGTACCGACCTCAGTAGCCGTGACTATCGACTCGTTATCAACCTGGGTCCGAatcagtaaagggtagcatgg
 Vβ 22gtttccctcttttctatgatAGAGTGTACCGACCTCAGTAGCCGTGACTATCGACTCAATCTAGTATCAGTGGCGCtatatcatttcagagtggct
 Vβ 23ctgagatttcattattataaAGAGTGTACCGACCTCAGTAGCCGTGACTATCGACTCCGTGTGTGCAGAGCCTATTattgaatatttcagacttct
 Vβ 24aacctggtatcttgggttctAGAGTGTACCGACCTCAGTAGCCGTGACTATCGACTCTGCCTGCTGTAGACTCGTAactagcttttcaaactgggt
PCR primers and hybridization control
Sequence, 5′-3′
Forward primerTAC TGA GGT CGG TAC ACT CT
Reverse primerTAMRA2-AGT AGC CGT GAC TAT CGA CT
Hybridization oligonucleotideTAMRA-TTT TTT GAC CGC GGA AGG GCT T
Hybridization control spot oligonucleotideAAG CCC TTC AGC GGT CAA AAA A-(TTC)5-T-NH2