Table 1.

Primers and probes for the real-time PCRs for RHD, RASSF1A, beta-actin, and SRY.

TargetNameSequencePrimer/probe
RASSF1ARSF-b151F5′-AGC CTG AGC TCA TTG AGC TG-3′Primer
RASSF1ARSF-dsgnR5′-ACC AGC TGC CGT GTG G-3′Primer
RASSF1ARSF-dsgnT5′-FAM-CCA ACG CGC TGC GCA T(MGB)-3′1Probe
beta-actinActin-163F5′-GCG CCG TTC CGA AAG TT-3′Primer
beta-actinActin-298R5′-CGG CGG ATC GGC AAA-3′Primer
beta-actinActin-243T5′-FAM-ACC GCC GAG ACC GCG TC(MGB)-3′Probe
SRYSRY-109F5′-TGG CGA TTA AGT CAA ATT CGC-3′Primer
SRYSRY-245R5′-CCC CCT AGT ACC CTG ACA ATG TAT T-3′Primer
SRYSRY-142T5′-FAM-AGCAGT AGA GCA GTC AGG GAG GCA GA(TAMRA)-3′Probe