Table 2. Method comparisons between nephelometry and LC-MRM/MS for the quantification of apoA-I and apoB.a
Protein and calibrationDenaturantSlopeIntercept
ApoA-I
    Calibration curveTFE2.10−78.1
Urea0.89−3.4
    Single calibratorTFE1.061.68
Urea0.9410.9
ApoB
    Calibration curveTFE1.26−5.2
Urea5.37−132.9
    Single calibratorTFE1.05−8.8
Urea1.13−2.1
  • a Deming regression coefficients are presented for the method comparison of immunoassays and LC-MRM/MS assays, which were performed with 2 different denaturation reagents and 2 different approaches to calibration.