Table 1.

Comparing the percentage of false-positive detection and true-positive detection of SABER at different terminator concentrations (A) and annealing temperatures (B).

 A. Effect of annealing temperature in the base extension step on the percentage of positive call. 52 °C* 54 °C 56 °C 58 °C 60 °C 62 °C 64 °C 66 °C 68 °C 70 °C True positive 100% 100% 100% 100% 100% 100% 87.5% 100% 87.5% 62.5% False positive 100% 100% 100% 87.5% 100% 75% 12.5% 0% 0% 0% B. Effect of terminator concentration on the percentage of positive call. Standard 2× 8× 20× 50× 100× True positive 100% 100% 100% 87.5% 100% 100% False positive 100% 100% 62.5% 12.5% 0% 0%
• 1 The percentage of positive call was calculated similarly as described in Fig. 1B .