Table 2.

Observed melting temperatures of IC amplicon, HSV-1 and -2 amplicon, and unlabeled probe HSVP/HSV ssDNA duplex.

Amplicon or probeDuplex length, bpMean melting temperature, °C1SD melting temperature, °CMaximum-minimum range, °C
IC amplicon (HSV-1)6476.040.220.65
IC amplicon (HSV-2)6476.000.090.27
HSV-1 amplicon15388.690.090.54
HSV-2 amplicon15387.650.180.24
HSVP detecting HSV-13771.600.310.93
HSVP detecting HSV-23783.030.150.44