Table 1.

Stability of the minor ethanol metabolites EtG and EtS during storage of infected urine specimens.

Test conditionsUropathogen identified
E. coli, nK. pneumoniae, nE. cloacae, n
Infected urine specimens incubated with 1.0 g/L ethanol for 5 days13666
Specimens initially positive for EtG and EtS21014
EtG unchanged after 5 days514
EtG negative or decreased after 5 days500
EtS unchanged after 5 days1014
EtS negative or decreased after 5 days000
Specimens initially negative for EtG and EtS2652
EtG negative after 5 days1752
EtG positive after 5 days3900
EtS negative after 5 days2652
EtS positive after 5 days000
  • 1 Ethanol was added to fresh urine specimens with confirmed growth of E. coli, K. pneumoniae, or E. cloacae and stored in sealed plastic vials without preservative at 22 °C for 5 days.

  • 2 Samples initially positive for EtG and EtS indicated that these patients had recently consumed alcohol. EtG and EtS were measured by LC-MS and the detection limit was approximately 0.1 mg/L for both compounds.

  • 3 All positive LC-MS results were confirmed positive by liquid chromatography-tandem mass spectrometry.