|Test conditions||Uropathogen identified|
|E. coli, n||K. pneumoniae, n||E. cloacae, n|
|Infected urine specimens incubated with 1.0 g/L ethanol for 5 days1||36||6||6|
|Specimens initially positive for EtG and EtS2||10||1||4|
|EtG unchanged after 5 days||5||1||4|
|EtG negative or decreased after 5 days||5||0||0|
|EtS unchanged after 5 days||10||1||4|
|EtS negative or decreased after 5 days||0||0||0|
|Specimens initially negative for EtG and EtS||26||5||2|
|EtG negative after 5 days||17||5||2|
|EtG positive after 5 days3||9||0||0|
|EtS negative after 5 days||26||5||2|
|EtS positive after 5 days||0||0||0|
1 Ethanol was added to fresh urine specimens with confirmed growth of E. coli, K. pneumoniae, or E. cloacae and stored in sealed plastic vials without preservative at 22 °C for 5 days.
2 Samples initially positive for EtG and EtS indicated that these patients had recently consumed alcohol. EtG and EtS were measured by LC-MS and the detection limit was approximately 0.1 mg/L for both compounds.
3 All positive LC-MS results were confirmed positive by liquid chromatography-tandem mass spectrometry.