Table 1.

Extracellular candidate tumor markers identified in culture medium of the PC3 (AR)6 roller bottle culture.

GeneProtein DescriptionMascot ScoreNo. Peptides Identified
GDF15Prostate differentiation factor; PTGF-β40.811
COL2A1Alpha 1 type II collagen, isoform 1, preproprotein; collagen II,44.962
PAM1Peptidylglycine α-amidating monooxygenase, isoform c, preproprotein45.642
IGFBP2Insulin-like growth factor binding protein 2 precursor (IGFBP-2) (IBP-2)45.71
S100A81S100 calcium-binding protein A8; cystic fibrosis antigen; calgranulin A47.91
TLR91Toll-like receptor 9, isoform A precursor49.442
TPT11Tumor protein, translationally controlled 150.731
CTSL1Cathepsin L52.091
PLGPlasminogen54.922
LGALS3Lectin, galactoside-binding, soluble, 3 (galectin 3); Lectin, galactose-binding55.531
LTBP21Latent transforming growth factor β binding protein 265.362
MUC5B1Mucin 5, subtype B, tracheobronchial66.332
GALNT61Polypeptide N-acetylgalactosaminyltransferase 666.352
IGFBP6Insulin-like growth factor binding protein 672.261
INHBBInhibin β B subunit precursor; Inhibin, β-274.861
TIMP2Tissue inhibitor of metalloproteinase 2 precursor75.212
LAMC2Laminin, γ 2, isoform a precursor; nicein (100 kDa)75.961
CXCL31Macrophage inflammatory protein-2-β precursor (MIP2-β)80.82
AZGP1α-2-glycoprotein 1, zinc; α-2-glycoprotein82.292
NPC21Niemann-Pick disease, type C2; Niemann-Pick disease, type C2 gene82.82
MDK1Midkine (neurite growth-promoting factor 2)90.342
SERPINA1Serine (or cysteine) proteinase inhibitor93.923
KLK61Kallikrein 6 (neurosin, zyme); protease M; protease, serine, 9 (neurosin)95.712
SEMA3F1Semaphorin 3F precursor (Semaphorin IV) (Sema IV) (Sema III/F)100.22
C19orf101Chromosome 19 open reading frame 10; interleukin 25; interleukin 27103.182
LRG1Leucine-rich α-2-glycoprotein109.663
COL6A2α 2 type VI collagen, isoform 2C2109.733
GALNT2Polypeptide N-acetylgalactosaminyltransferase 2; UDP-GalNAc transferase 2114.53
IGFBP5Insulin-like growth factor binding protein 5120.383
RNASET21Ribonuclease 6 (EC 3.10.27.—) precursor124.534
FBLN1Fibulin 1 isoform C precursor137.33
DAG11Dystroglycan 1 precursor; α-dystroglycan; Dystrophin-associated glycoprotein-1145.923
S100A9S100 calcium-binding protein A9; calgranulin B152.13
TGFB2Transforming growth factor, β 2152.593
SPINT11Hepatocyte growth factor activator inhibitor precursor154.243
B2 mol/Lβ-2-microglobulin177.473
LCN21Lipocalin 2 (oncogene 24p3)181.585
KLK51Kallikrein 5; stratum corneum tryptic enzyme181.944
LCN71P3ECSL; glucocorticoid-inducible protein; oxidized-LDL responsive gene 2184.673
DFComplement factor D preproprotein; adipsin; properdin factor D187.964
SFN1Stratifin193.84
APP1Amyloid β (A4) precursor protein (protease nexin-II, Alzheimer disease)215.476
TIMP1Tissue inhibitor of metalloproteinase 1 precursor; Erythroid-potentiating activity227.554
C1SComplement component 1, s subcomponent254.894
GRNGranulin258.535
PLTP1Phospholipid transfer protein282.025
STC21Stanniocalcin 2; stanniocalcin 2; stanniocalcin-related protein3034
PTX31Pentaxin-related gene, rapidly induced by IL-1β; Pentraxin-3308.445
CYR611Cysteine-rich, angiogenic inducer, 61; cysteine-rich heparin-binding protein 61327.56
IL6Interleukin 6 (interferon, β 2)336.166
PSAP1Saposin precursor359.659
PLAUPlasminogen activator, urokinase361.26
FSTL11Follistatin-like 1 precursor; follistatin-related protein367.117
P4HB1Procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase)375.338
AGRN1Agrin486.699