Table 1.

Step-wise validation results for a selected subset of 25 marker candidates derived from the discovery process and from the literature.1

Gene symbol (HUGO)Methylation arrayReal-time PCR
AUC, CRC vs all controlsAUC, CRC vs colon controlsPBL: median PMR, %PBL: median PMR, %Healthy colon: median PMR (90% range), %CRC: median PMR, (90% range), %AUCExclusion criteria
BCL60.730.7825Failed chip-selection criteria
DNAJC50.670.7323
DUX20.750.7017
ESTG02089620.640.639
MSH630.580.640
ESTG230860920.620.5622
DLX50.840.830PBL+Failed PBL filter
NPBWR1 (GPR7)0.730.820PBL+
APBA23NT4NTNTPBL+
SLC32A10.850.8901.0
SLITRK10.860.8510.3
KCTD120.670.7700.00 (0–0)0 (0–28)0.53Insufficient CRC methylation
ONECUT20.720.7720.01 (0–1)0 (0–2)0.24
RNF40.790.8400.00 (0–0)0 (0–0)0.52
SMAD70.680.7100.00 (0–0)0 (0–1)0.59
TUSC330.820.7410.23 (1–10)11 (1–32)0.71Failed LOD test
TMEFF20.900.9400.14 (1–9)30 (1–81)0.81
ZDHHC220.880.8790.00 (0–1)3 (0–27)0.78HM assay failed
VTN0.93NT10.02 (0–4)20 (0–77)0.81Plasma positive
FOXL20.67NT00.0NT7 (0–38)NTCandidates for plasma testing
NGFR0.830.8700.00 (0–2)3 (0–60)0.69
ALX40.650.8300.11 (0–2)11 (0–58)0.74
SIX60.770.8500.00 (0–0)16 (0–46)0.83
EYA40.660.8140.02 (0–4)26 (0–76)0.88
SEPT90.84NT00.02 (1–7)26 (0–89)0.87