Table 3.

Plasma performance of single markers.

TMEFF2NGFRSEPT9bisDNA
Assay LOD in background of 50 ng PBL DNA, pg/reaction702119
Positives, %
 Normal plasma1311614100
 CRC plasma655169100
Median DNA (90% range), mg/L
 Normal plasma0 (0–0.078)0 (0–0.017)0 (0–0.010)2.7 (0.7–112.3)
 CRC plasma0.010 (0–3.316)0 (0–0.937)0.013 (0–4.105)3.5 (0.6–498.4)
AUC0.720.700.800.61
P0000.0001
Cutoff for 95% specificity for normal plasma (n = 179), mg/L0.0980.0190.011150.3
Sensitivity at 95% specificity by CRC stage, %
 All stages (n = 133)230335215
 Stage 0/I (n = 20)520300
 Stage II (n = 32)22255616
 Stage III (n = 47)34364521
 Stage IV (n = 31)45366816