Table 1.

Primers, probes, and their corresponding annealing temperatures for both conventional and real-time quantitative PCR.

GeneSequenceForward/reverse/probeAmplicon size, bpAnnealing temperature, °C
leptin5′-CAGTCTCCTCCAAACAGAAAGTCA-3′Forward1
5′-GTCCATCTTGGATAAGGTCAGGA-3′Reverse10558
5′-GATATTTGGATCACGTTTCTGG-3′Reverse14558
5′-CAGCTCTTAGAGAAGGCCAGCA-3′Reverse20158
5′-CTCTGTGGAGTAGCCTGAAGCTT-3′Reverse28058
5′-CTCAGCACCCAGGGCTGAG-3′Reverse35658
5′-GTGTCCATGCAATGCTCTTCA-3′Reverse44960
5′-CCTTCCTGGTGAGAATAGGATCC-3′Reverse57660
5′-GGTCACATTGAGGCATTCAGTGAG-3′Reverse69760
5′-GGATGTGGTCTTCATGGAACTCCT-3′Reverse79860
5′-(FAM)2CGGTTTGGACTTC(MGBNFQ)-3′Probe1
SRY5′-AAAGGCAACGTCCAGGATAGAG-3′Forward1
5′-TGAGTTTCGCATTCTGGGATT-3′Reverse10758
5′-CCACTGGTATCCCAGCTGCT-3′Reverse13758
5′-TGTAATTTCTGTGCCTCCTGGA-3′Reverse19358
5′-ACTTCGCTGCAGAGTACCGAA-3′Reverse31358
5′-TAAGTGGCCTAGCTGGTGCTC-3′Reverse39258
5′-ATGTTACCCGATTGTCCTACAGC-3′Reverse52458
5′-(FAM)AAGCGACCCATGAA(MGBNFQ)-3′Probe1