Table 1.

Capture probes used and cell lines harboring corresponding mutations.

No.Mutation (codon)Cell lineCapture probe1
 1Wild type (Gly12/13)Colo320  CTGGTGGCGTAGG
 2Ala12SW1116 AGCTGCTGGCGTA
 3Arg12H157 AGCTCGTGGCGTA
 4Asp12LS174T AGCTGATGGCGTA
 5Asp13DLD1  TGGTGACGTAGGC
 6Cys12MIA Paca2 AGCTTGTGGCGTA
 7Cys13H1355  TGGTTGCGTAGGC
 8Ile12Not available2GAGCTATTGGCGT
 9Leu12Not available2GAGCTCTTGGCGT
10Ser12A549 AGCTAGTGGCGTA
11Val12SW480 AGCTGTTGGCGTA
12(HLA-)DRA PMAllGGAGACGGTCTGG
13(HLA-)DRAMMGGAGACGCTCTGG
14PCR control3AllAAGGCCTGCTGAA
15Hybridization control PMSynthetic targetACAGAAACGAGCG
16Hybridization controlMMACAGAAACCAGCG