Table 1.

Comparison of the predictive performance of different equations for the calculation of oligonucleotide Tm.1

N-N calculationsEquation
Including mismatchesWithout mismatches1 22 29 10 11 12
n475217131200217217217217
r20.900.890.780.600.860.870.880.88
Slope1.001.051.200.951.010.960.971.01
Intercept, ΔTm−0.29−3.17−18.445.83−0.602.652.32−0.54
Sy|x2.061.762.213.131.991.881.871.86
Mean ± SD of differences (observed minus predicted)0.1 ± 2.180.2 ± 1.966.1 ± 3.13−2.5 ± 3.84−0.1 ± 1.930.0 ± 2.16−0.1 ± 2.000.1 ± 2.18