Table 1.

Concordance rate listed by site and genotype and protocol variables listed by site.

Wild typeGenotypeAll genotypesDNA extraction methodFluorescence methodReference method
HeterozygoteMutant
SiteStop and transferDirectStop and transferDirectStop and transferDirectStop andtransferDirect
1101/101ND12/2ND1/1ND104/104NDEpicentre2 or Qiagen3Dynex FluorolitePCR-RFLP
2201/201201/20118/1818/181/11/1220/220220/220Qiagen3CytoFluor 4000AS-PCR
388/8888/887/77/71/11/196/9696/96Qiagen3CytoFluor 4000PCR-RFLP
494/94ND7/7ND1/1ND102/102NDBio-Rad4Dynex FluorolitePCR-RFLP
Total484/484289/28934/3425/254/42/2522/522316/316
  • 1 ND, not determined.

  • 2 Epicentre MasterPure DNA Purification Kit.

  • 3 Qiagen QIAamp Blood Kit.

  • 4 Bio-Rad InstaGene Whole Blood Kit.