Table 1.

Total and intraassay imprecision (CV) for measurement of transcobalamin by ELISA.1

Assigned value, pmol/LMeasured mean, pmol/LEstimates (runs), nTotal CV, %Intraassay CV, %
66.77353 (27)4.02.3
53.35555 (27)4.42.5
40.03952 (25)6.22.4
26.72654 (27)5.82.6
13.313.754 (27)4.12.9
  • 1 A pool of human plasma diluted 1:20 with assay buffer (S1) was prepared and diluted to contain 80%, 60% (S3), 40%, and 20% S1. The transcobalamin concentration in S3 was assigned to be 40 pmol/L, and the concentrations in the other pools were calculated from this value. The samples were analyzed in duplicate in 27 independent assays over a period of 2 months, using three different batches of coated microtiter plates.