Table 1.

Common clinical uses of flow cytometry.

FieldClinical applicationCommon characteristic measured
ImmunologyHistocompatibility cross-matchingIgG, IgM
Transplantation rejectionCD3, circulating OKT3
HLA-B27 detectionHLA-B27
Immunodeficiency studiesCD4, CD8
OncologyDNA content and S phase of tumorsDNA
Measurement of proliferation markersKi-67, PCNA1
HematologyLeukemia and lymphoma phenotypingLeukocyte surface antigens
Identification of prognostically important subgroupsTdT, MPO
Hematopoietic progenitor cell enumerationCD34
Diagnosis of systemic mastocytosisCD25, CD69
Reticulocyte enumerationRNA
Autoimmune and alloimmune disorders
Anti-platelet antibodiesIgG, IgM
Anti-neutrophil antibodiesIgG
Immune complexesComplement, IgG
Feto-maternal hemorrhage quantificationHemoglobin F, rhesus D
Blood bankingImmunohematologyErythrocyte surface antigens
Assessment of leukocyte contamination of blood productsForward and side scatter, leukocyte surface antigens
Genetic disordersPNHCD55, CD59
Leukocyte adhesion deficiencyCD11/CD18 complex