Table 2.

Oligonucleotides used in this study.

OligonucleotidesNucleotide sequences
GBS-specific primers1
Sag595′-TTTCACCAGCTGTATTAGAAGTA-3′
Sag1905′-GTTCCCTGAACATTATCTTTGAT-3′
Adjacent hybridization probes specific for the GBS amplicon
STB-F5′-AAGCCCAGCAAATGGCTCAAA-fluorescein-3′
STB-C5′-Cy5-GCTTGATCAAGATAGCATTCAGTTGA-phosphate-3′
Adjacent hybridization probes specific for the internal control amplicon
IC-F5′-TTATTGCAGCTTCGCCACAGGAA-fluorescein-3′
IC-C5′-Cy5-GGTCCAGCAATGTGAAGAGGCAT-phosphate-3′