RT Journal Article SR Electronic T1 Discovery and Validation of Salivary Extracellular RNA Biomarkers for Noninvasive Detection of Gastric Cancer JF Clinical Chemistry JO Clin. Chem. FD American Association for Clinical Chemistry SP 1513 OP 1521 DO 10.1373/clinchem.2018.290569 VO 64 IS 10 A1 Li, Feng A1 Yoshizawa, Janice M. A1 Kim, Kyoung-Mee A1 Kanjanapangka, Julie A1 Grogan, Tristan R. A1 Wang, Xiaoyan A1 Elashoff, David E. A1 Ishikawa, Shigeo A1 Chia, David A1 Liao, Wei A1 Akin, David A1 Yan, Xinmin A1 Lee, Min-Sun A1 Choi, Rayun A1 Kim, Su-Mi A1 Kang, So-Young A1 Bae, Jae-Moon A1 Sohn, Tae-Sung A1 Lee, Jun-Ho A1 Choi, Min-Gew A1 Min, Byung-Hoon A1 Lee, Jun-Haeng A1 Kim, Jae J. A1 Kim, Yong A1 Kim, Sung A1 Wong, David T.W. YR 2018 UL http://clinchem.aaccjnls.org/content/64/10/1513.abstract AB BACKGROUND: Biomarkers are needed for noninvasive early detection of gastric cancer (GC). We investigated salivary extracellular RNA (exRNA) biomarkers as potential clinical evaluation tools for GC.METHODS: Unstimulated whole saliva samples were prospectively collected from 294 individuals (163 GC and 131 non-GC patients) who underwent endoscopic evaluation at the Samsung Medical Center in Korea. Salivary transcriptomes of 63 GC and 31 non-GC patients were profiled, and mRNA biomarker candidates were verified with reverse transcription quantitative real-time PCR (RT-qPCR). In parallel, microRNA (miRNA) biomarkers were profiled and verified with saliva samples from 10 GC and 10 non-GC patients. Candidate biomarkers were validated with RT-qPCR in an independent cohort of 100/100 saliva samples from GC and non-GC patients. Validated individual markers were configured into a best performance panel.RESULTS: We identified 30 mRNA and 15 miRNA candidates whose expression pattern associated with the presence of GC. Among them, 12 mRNA and 6 miRNA candidates were verified with the discovery cohort by RT-qPCR and further validated with the independent cohort (n = 200). The configured biomarker panel consisted of 3 mRNAs (SPINK7, PPL, and SEMA4B) and 2 miRNAs (MIR140-5p and MIR301a), which were all significantly down-regulated in the GC group, and yielded an area under the ROC curve (AUC) of 0.81 (95% CI, 0.72–0.89). When combined with demographic factors, the AUC of the biomarker panel reached 0.87 (95% CI, 0.80–0.93).CONCLUSIONS: We have discovered and validated a panel of salivary exRNA biomarkers with credible clinical performance for the detection of GC. Our study demonstrates the potential utility of salivary exRNA biomarkers in screening and risk assessment for GC.