RT Journal Article
SR Electronic
T1 Soluble Donor DNA Concentrations in Recipient Serum Correlate with Pancreas-Kidney Rejection
JF Clinical Chemistry
JO Clin. Chem.
FD American Association for Clinical Chemistry
SP 379
OP 382
DO 10.1373/clinchem.2005.058974
VO 52
IS 3
A1 Gadi, Vijayakrishna K.
A1 Nelson, J. Lee
A1 Boespflug, Nicholas D.
A1 Guthrie, Katherine A.
A1 Kuhr, Christian S.
YR 2006
UL http://clinchem.aaccjnls.org/content/52/3/379.abstract
AB Background: There is no reliable serum marker available to monitor incipient pancreas or islet-cell rejection. We tested the hypothesis that quantification of donor-specific genomic DNA in serum (from tissue damage) can serve as a marker of rejection. Methods: Using a recently developed panel of HLA-specific quantitative PCR assays (Q-PCR), we tested 158 sera from 42 pancreas-kidney transplant recipients. Temporally related biopsies for 65 sera permitted analysis for correlation of donor DNA concentrations with rejection. Results: Donor DNA concentrations were higher in sera from recipients who had experienced allograft rejection (n = 31) than from those who had not (n = 34). Median concentrations, expressed as the genome-equivalent (gEq) number of donor cells per 106 host cells, were 2613 and 59 gEq/106, respectively (P = 0.03). Conclusion: Q-PCR for donor-specific genetic polymorphisms merits further investigation as a noninvasive approach to monitor pancreas-kidney as well as other types of allograft rejection.