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Research ArticlePoint-of-Care Testing

Screening Method to Evaluate Point-of-Care Human Chorionic Gonadotropin (hCG) Devices for Susceptibility to the Hook Effect by hCG β Core Fragment: Evaluation of 11 Devices

Robert D. Nerenz, Haowei Song, Ann M. Gronowski
DOI: 10.1373/clinchem.2013.217661 Published January 2014
Robert D. Nerenz
Department of Pathology and Immunology,
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Haowei Song
Department of Medicine, Washington University School of Medicine, St. Louis, MO.
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Ann M. Gronowski
Department of Pathology and Immunology,
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Abstract

Background: The predominant hCG variant in urine, hCG β core fragment (hCGβcf), has been demonstrated to cause false-negative results in qualitative point-of-care (POC) hCG devices. This is a major concern for healthcare professionals using POC pregnancy tests. We developed a screening method to evaluate qualitative POC hCG devices for their susceptibility to inhibition by hCGβcf. Using this method, we evaluated the performance of 11 commonly used devices.

Methods: A wide range of purified hCG and hCGβcf concentrations were mixed and tested on 2 POC devices. By use of those results, a screening method was defined and 9 additional POC devices were evaluated. Two solutions containing (a) 500 pmol/L (171 IU/L) intact hCG with 0 pmol/L hCGβcf and (b) 500 pmol/L intact hCG with 500 000 pmol/L hCGβcf were used to screen all POC devices.

Results: The OSOM and Cen-Med Elite devices were found to be most susceptible to false-negative results due to hCGβcf. The BC Icon 20 and the Alere were the least susceptible. The remaining 7 were moderately affected. Devices that gave the strongest signal with hCGβcf alone were those that were least likely to show a hook effect.

Conclusions: The screening method put forth here can be used by device users and manufacturers to evaluate POC devices for inhibition by hCGβcf. Of 11 devices evaluated, only 2 have been identified that exhibit minimal to no susceptibility to hCGβcf.

  • Received for publication October 15, 2013.
  • Accepted for publication January 7, 2014.
  • © 2014 The American Association for Clinical Chemistry
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Clinical Chemistry: 65 (2)
Vol. 65, Issue 2
February 2019
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Screening Method to Evaluate Point-of-Care Human Chorionic Gonadotropin (hCG) Devices for Susceptibility to the Hook Effect by hCG β Core Fragment: Evaluation of 11 Devices
Robert D. Nerenz, Haowei Song, Ann M. Gronowski
Clinical Chemistry Jan 2014, clinchem.2013.217661; DOI: 10.1373/clinchem.2013.217661
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Screening Method to Evaluate Point-of-Care Human Chorionic Gonadotropin (hCG) Devices for Susceptibility to the Hook Effect by hCG β Core Fragment: Evaluation of 11 Devices
Robert D. Nerenz, Haowei Song, Ann M. Gronowski
Clinical Chemistry Jan 2014, clinchem.2013.217661; DOI: 10.1373/clinchem.2013.217661

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