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Research ArticleMolecular Diagnostics and Genetics

Magnetically Promoted Rapid Immunoreactions Using Functionalized Fluorescent Magnetic Beads: A Proof of Principle

Satoshi Sakamoto, Kenshi Omagari, Yoshinori Kita, Yusuke Mochizuki, Yasuyuki Naito, Shintaro Kawata, Sachiko Matsuda, Osamu Itano, Hiromitsu Jinno, Hiroya Takeuchi, Yuki Yamaguchi, Yuko Kitagawa, Hiroshi Handa
DOI: 10.1373/clinchem.2013.211433 Published January 2014
Satoshi Sakamoto
Department of Biological Information, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Japan;
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Kenshi Omagari
Department of Surgery, School of Medicine, Keio University, Tokyo, Japan.
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Yoshinori Kita
Department of Biological Information, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Japan;
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Yusuke Mochizuki
Department of Biological Information, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Japan;
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Yasuyuki Naito
Department of Biological Information, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Japan;
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Shintaro Kawata
Department of Biological Information, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Japan;
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Sachiko Matsuda
Department of Surgery, School of Medicine, Keio University, Tokyo, Japan.
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Osamu Itano
Department of Surgery, School of Medicine, Keio University, Tokyo, Japan.
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Hiromitsu Jinno
Department of Surgery, School of Medicine, Keio University, Tokyo, Japan.
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Hiroya Takeuchi
Department of Surgery, School of Medicine, Keio University, Tokyo, Japan.
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Yuki Yamaguchi
Department of Biological Information, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Japan;
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Yuko Kitagawa
Department of Surgery, School of Medicine, Keio University, Tokyo, Japan.
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Hiroshi Handa
Department of Biological Information, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Japan;
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Abstract

Background: Accurate detection and monitoring of disease-related biomarkers is important in understanding pathophysiology. We devised a rapid immunoreaction system that uses submicrometer polymer-coated fluorescent ferrite (FF) beads containing both ferrites (magnetic iron oxide) and fluorescent europium complexes.

Methods: FF beads were prepared by encapsulation of hydrophobic europium complexes into the polymer layers of affinity magnetic matrices using organic solvent. A sandwich immunoassay using magnetic collection of antibody-coated FF beads to a specific place was performed. Brain natriuretic peptide and prostate-specific antigen were selected as target detection antigens to demonstrate the feasibility of this approach. An immunohistochemical staining using magnetic collection of antibody-coated FF beads onto carcinoma cell samples was also performed.

Results: The sandwich immunoassays, taking advantage of the magnetic collection of antibody-coated FF beads, detected target antigens within 5 min of sample addition. Without magnetic collection, the sandwich immunoassay using antibody-coated FF beads required long times to obtain results, similar to conventional immunoassays. With the use of magnetic collection of antibody-coated FF beads, immunohistochemical staining enabled discrimination of carcinoma cells within 20 min.

Conclusions: This proof of principle system demonstrates that immunoreactions involving the magnetic collection of antibody-coated FF beads allow acceleration of the antigen–antibody reaction. The simple magnetic collection of antibody-coated FF beads to a specific space enables rapid detection of disease-related biomarkers and identification of carcinoma cells.

  • Received for publication July 10, 2013.
  • Accepted for publication December 2, 2013.
  • © 2013 The American Association for Clinical Chemistry
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Clinical Chemistry: 65 (2)
Vol. 65, Issue 2
February 2019
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Magnetically Promoted Rapid Immunoreactions Using Functionalized Fluorescent Magnetic Beads: A Proof of Principle
Satoshi Sakamoto, Kenshi Omagari, Yoshinori Kita, Yusuke Mochizuki, Yasuyuki Naito, Shintaro Kawata, Sachiko Matsuda, Osamu Itano, Hiromitsu Jinno, Hiroya Takeuchi, Yuki Yamaguchi, Yuko Kitagawa, Hiroshi Handa
Clinical Chemistry Jan 2014, clinchem.2013.211433; DOI: 10.1373/clinchem.2013.211433
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Magnetically Promoted Rapid Immunoreactions Using Functionalized Fluorescent Magnetic Beads: A Proof of Principle
Satoshi Sakamoto, Kenshi Omagari, Yoshinori Kita, Yusuke Mochizuki, Yasuyuki Naito, Shintaro Kawata, Sachiko Matsuda, Osamu Itano, Hiromitsu Jinno, Hiroya Takeuchi, Yuki Yamaguchi, Yuko Kitagawa, Hiroshi Handa
Clinical Chemistry Jan 2014, clinchem.2013.211433; DOI: 10.1373/clinchem.2013.211433

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