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LetterLetters to the Editor

Robust and Accurate 2-Year Performance of a Quantitative Mass Spectrometry-Based Apolipoprotein Test in a Clinical Chemistry Laboratory

L. Renee Ruhaak, Nico P.M. Smit, Fred P.H.T.M. Romijn, Mervin M. Pieterse, Arnoud van der Laarse, Yuri E.M. van der Burgt, Christa M. Cobbaert
DOI: 10.1373/clinchem.2017.285098 Published March 2018
L. Renee Ruhaak
Department of Clinical Chemistry and Laboratory Medicine Leiden University Medical Center Leiden, the Netherlands
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Nico P.M. Smit
Department of Clinical Chemistry and Laboratory Medicine Leiden University Medical Center Leiden, the Netherlands
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Fred P.H.T.M. Romijn
Department of Clinical Chemistry and Laboratory Medicine Leiden University Medical Center Leiden, the Netherlands
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Mervin M. Pieterse
Department of Clinical Chemistry and Laboratory Medicine Leiden University Medical Center Leiden, the Netherlands
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Arnoud van der Laarse
Department of Clinical Chemistry and Laboratory Medicine Leiden University Medical Center Leiden, the Netherlands
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Yuri E.M. van der Burgt
Department of Clinical Chemistry and Laboratory Medicine Leiden University Medical Center Leiden, the Netherlands
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Christa M. Cobbaert
Department of Clinical Chemistry and Laboratory Medicine Leiden University Medical Center Leiden, the Netherlands
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  • For correspondence: c.m.cobbaert@lumc.nl
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To the Editor:

We previously described a multiplex liquid chromatography–mass spectrometry (LC-MS)1 method for the quantification of 6 apolipoproteins (apo): A-I, B, C-I, C-II, C-III, and E (1). Analytical method validation was performed according to the Clinical and Laboratory Standards Institute (CLSI) protocols, and the intraassay CVs were 2.3% to 5.5% and total CVs were 2.5% to 5.9%. Moreover, results were interchangeable with immunoturbidimetric assays for apo A-I (Deming slope, 1.014) and for apo B-100 (Deming slope, 1.016) (1). Our laboratory-developed test has now been used intermittently during a 2-year period in various studies on clinical cohorts. Here we demonstrate the robustness and analytical performance of mass spectrometry-based apolipoprotein quantification using stable isotope dilution and multiple reaction monitoring (SID-MRM) on a triple quadrupole for routine practice in clinical laboratories.

To evaluate performance of the LC-MS test with regard to precision and bias of test results, 2 native serum pools were included as internal quality control (QC) on each of the 96-well plate batches analyzed (both in triplicate) over this period. The measurements were compared with assigned values to assess bias and precision by analysis of repeated QC observations in …

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Clinical Chemistry: 64 (4)
Vol. 64, Issue 4
April 2018
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Robust and Accurate 2-Year Performance of a Quantitative Mass Spectrometry-Based Apolipoprotein Test in a Clinical Chemistry Laboratory
L. Renee Ruhaak, Nico P.M. Smit, Fred P.H.T.M. Romijn, Mervin M. Pieterse, Arnoud van der Laarse, Yuri E.M. van der Burgt, Christa M. Cobbaert
Clinical Chemistry Apr 2018, 64 (4) 747-749; DOI: 10.1373/clinchem.2017.285098
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Robust and Accurate 2-Year Performance of a Quantitative Mass Spectrometry-Based Apolipoprotein Test in a Clinical Chemistry Laboratory
L. Renee Ruhaak, Nico P.M. Smit, Fred P.H.T.M. Romijn, Mervin M. Pieterse, Arnoud van der Laarse, Yuri E.M. van der Burgt, Christa M. Cobbaert
Clinical Chemistry Apr 2018, 64 (4) 747-749; DOI: 10.1373/clinchem.2017.285098

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