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Abstract

Pre-precipitated and solid-phase second antibody compared in radioimmunoassay.

T R Brown, N Bagchi, T T Ho, R E Mack
Published March 1980
T R Brown
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N Bagchi
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T T Ho
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R E Mack
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Abstract

We describe simple methods for preparing and determining the titer of pre-precipitated second antibody. We compared the performance of this type of insolubilized second antibody with that of a commercially available solid-phase second antibody (DASP) for separation of reverse triiodothyronine (rT3) and for thyrotropin radioimmunoassays. Equilibrium time for both reagents was about 30 min, considerably briefer than for the usual second-antibody procedure. Within-assay precision was improved and percentages of nonspecific binding (mean and SD) were lower if pre-precipitated second antibody was used in both assays [rT3 pre-precipitate = 1.48 (SD 0.16), rT3 DASP = 3.76 (SD 0.22); thyrotropin pre-precipitate = 1.45 (SD 0.18), thyrotropin DASP = 3.45 (SD 0.26)]. The cost of DASP was three to 10 times that of pre-precipitated second antibody prepared with commercially available products.

  • © 1980 The American Association for Clinical Chemistry
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Vol. 26, Issue 3
March 1980
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Pre-precipitated and solid-phase second antibody compared in radioimmunoassay.
T R Brown, N Bagchi, T T Ho, R E Mack
Clinical Chemistry Mar 1980, 26 (3) 503-507;
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Pre-precipitated and solid-phase second antibody compared in radioimmunoassay.
T R Brown, N Bagchi, T T Ho, R E Mack
Clinical Chemistry Mar 1980, 26 (3) 503-507;

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