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Abstract

Enzymic color development of urinary 3 alpha-hydroxysteroids on thin-layer chromatograms.

Y Yamaguchi
Published March 1980
Y Yamaguchi
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Abstract

I describe the use of 3 alpha-hydroxysteroid dehydrogenase (EC 1.1.1.50) for enzymic color development of 3 alpha-hydroxysteroids. Urinary 3 alpha-hydroxysteroids are converted to 3-ketosteroids by the enzyme, and the NADH formed is reacted with 2-p-iodophenyl-3-p-nitrophenyl-5-phenyl-tetrazolium chloride and "diaphorase" to form the colored formazan. The dye so formed on a thin layer chromatographic plate is measured by densitometoric scanning. I illustrate use of this method by showing results for urinary excretion of 3 alpha-hydroxysteroids by some various sorts of patients.

  • © 1980 The American Association for Clinical Chemistry
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Vol. 26, Issue 3
March 1980
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Enzymic color development of urinary 3 alpha-hydroxysteroids on thin-layer chromatograms.
Y Yamaguchi
Clinical Chemistry Mar 1980, 26 (3) 491-493;
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Enzymic color development of urinary 3 alpha-hydroxysteroids on thin-layer chromatograms.
Y Yamaguchi
Clinical Chemistry Mar 1980, 26 (3) 491-493;

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